The Ultimate Guide to 96 Deep Well Plates
96 deep well plates are essential tools in modern laboratories, providing efficient storage and handling for a wide range of samples. These plates are designed to optimize storage capacity, improve sample mixing, and facilitate various applications including combinatorial chemistry, sample preparation, and high-throughput screening. In this comprehensive guide, we will explore the different types of 96 deep well plates available in the market, their applications, and key technical features to help you make an informed decision for your laboratory needs.
Comparison of 96 Deep Well Plates
| Brand/Model | Capacity (mL) | Material | Well Type | Special Features | Applications |
|---|---|---|---|---|---|
| Thermo Scientific Nunc™ | 1.3, 2.0 | Polypropylene | U and V shaped | Shared-Wall Technology, low dead-volume | Storage, mixing, extraction |
| Eppendorf Deepwell Plates | 1.0 | Polypropylene | V shaped | OptiTrack™ matrix, g-Safe™ technology | Sample handling, centrifugation |
| VWR® Deep Well Plates | 1.0 | Polypropylene | Flat bottom | Stackable design | High-throughput applications |
| Agilent 96 & 384-Well Plates | 1.0 | Polypropylene | Various | Multiple formats available | Screening, sample preparation |
| Fisherbrand™ DeepWell Plates | 2.0 | Polypropylene | U shaped | Low-binding characteristics | Storage, transport |
| USA Scientific PlateOne® | 2.0 | Polypropylene | Square wells | Custom options available | Storage, assay setup |
| Axygen® Clear V-Bottom | 0.5 | Polypropylene | V shaped | Transparent for easy viewing | Sample preparation |
| BRAND® Stackable Plates | 1.0 | Polypropylene | Flat bottom | Space-saving stackable design | General laboratory use |
| Sigma-Aldrich 96 Deep Well | 2.1 | Polypropylene | Square wells | Robust design | High-throughput screening |
| Ops Diagnostics | 2.0 | Polypropylene | V shaped | Low dead-volume, high recovery | Sample storage, extraction |
Types of 96 Deep Well Plates
Polypropylene Plates
Polypropylene is the most common material used for 96 deep well plates due to its chemical resistance and low-binding properties. Brands like Thermo Scientific and Eppendorf utilize high-quality polypropylene to ensure reliable performance in various applications.
Specialized Plates
Some plates come with unique features such as OptiTrack™ matrix for quick identification (Eppendorf) or shared-wall technology (Thermo Scientific) that enhances mixing and reduces dead volume. These innovations cater to specific laboratory needs, making the selection process crucial.
Stackable Plates
Stackable designs, as offered by VWR and BRAND, allow for efficient storage in limited lab spaces. This feature is especially beneficial for high-throughput applications where multiple plates may be used simultaneously.
Applications of 96 Deep Well Plates
Sample Storage
Deep well plates are ideal for long-term storage of samples without contamination. Their low-binding characteristics, particularly in polypropylene plates, make them suitable for preserving sample integrity.
High-Throughput Screening
96 deep well plates facilitate high-throughput screening processes in drug discovery and genomics. Their design allows for simultaneous processing of multiple samples, which significantly accelerates research timelines.
Sample Preparation
These plates are extensively used for sample preparation, including dilutions and aliquots. The U and V shaped wells in Thermo Scientific plates, for instance, enhance mixing efficiency, leading to better sample preparation results.
Combinatorial Chemistry
In combinatorial chemistry, deep well plates provide an efficient means for storing and mixing various chemical compounds. Their resistance to solvents makes them ideal for diverse chemical applications.
Technical Features Comparison
| Feature | Thermo Scientific | Eppendorf | VWR | Agilent | Fisherbrand | USA Scientific | Axygen | BRAND | Sigma-Aldrich | Ops Diagnostics |
|———————————-|——————-|—————-|—————-|——————|—————-|—————-|—————-|—————-|——————|
| Material | Polypropylene | Polypropylene | Polypropylene | Polypropylene | Polypropylene | Polypropylene | Polypropylene | Polypropylene | Polypropylene | Polypropylene |
| Well Capacity (mL) | 1.3, 2.0 | 1.0 | 1.0 | 1.0 | 2.0 | 2.0 | 0.5 | 2.1 | 2.0 |
| Well Type | U and V shaped | V shaped | Flat bottom | Various | U shaped | Square wells | V shaped | Flat bottom | V shaped |
| Special Features | Low dead-volume | OptiTrack™, g-Safe™ | Stackable | Multiple formats | Low-binding | Custom options | Transparent | Space-saving | High recovery |
| Applications | Storage, mixing | Sample handling | High-throughput | Screening | Storage | Assay setup | Sample preparation | General use | Extraction |
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Conclusion
In summary, 96 deep well plates are indispensable tools in laboratory settings, designed to enhance sample handling, storage, and preparation across various applications. With options ranging from polypropylene materials to specialized features such as low dead-volume and stackable designs, choosing the right plate is vital for optimizing laboratory processes. Leading brands like www.thermofisher.com, www.eppendorf.com, and www.avantorsciences.com offer a wide array of choices tailored to meet diverse research requirements.
FAQ
What are 96 deep well plates used for?
96 deep well plates are primarily used for sample storage, high-throughput screening, and sample preparation in laboratories. They facilitate efficient handling and mixing of samples across various applications.
What material are deep well plates typically made from?
Most deep well plates are made from polypropylene due to its chemical resistance and low-binding properties, which help maintain sample integrity.
What is the capacity of a standard 96 deep well plate?
The capacity of a standard 96 deep well plate typically ranges from 0.5 mL to 2.1 mL, depending on the design and manufacturer.
What are U and V shaped wells?
U and V shaped wells refer to the shape of the bottom of the wells in the deep well plates. U shaped wells facilitate better mixing and sample extraction, while V shaped wells minimize dead volume.
Can deep well plates be used for long-term sample storage?
Yes, deep well plates are ideal for long-term sample storage, especially when made from low-binding polypropylene to prevent sample loss.
What is OptiTrack™ technology?
OptiTrack™ technology is a feature available in Eppendorf deep well plates that allows for rapid identification of samples, enhancing efficiency in laboratories.
Are there stackable deep well plates?
Yes, brands like VWR and BRAND offer stackable deep well plates that save space and improve organization in laboratory environments.
What is the significance of low dead-volume in deep well plates?
Low dead-volume is crucial as it ensures that a minimal amount of sample is left behind in the wells after processing, leading to higher recovery rates during experiments.
How do I choose the right deep well plate for my application?
Choosing the right deep well plate depends on factors such as sample type, required volume, and specific features like mixing efficiency. Consider your laboratory’s needs and consult product specifications.
Where can I purchase 96 deep well plates?
96 deep well plates can be purchased from various scientific suppliers, including www.thermofisher.com, www.eppendorf.com, and www.fishersci.com among others.
